Animal Cell Technology: Challenges for the 21st Century by Kouji Ikura, Masaya Nagao, Seiji Masuda, Ryuzo Sasaki PDF
By Kouji Ikura, Masaya Nagao, Seiji Masuda, Ryuzo Sasaki
Animal mobilephone know-how is a becoming self-discipline of mobile biology which goals not just to appreciate constructions, features and behaviors of differentiated animal cells but additionally to check their skills for use in commercial and scientific reasons. The objective of animal mobilephone know-how comprises accomplishments of clonal enlargement of differentiated cells with invaluable skill, optimization in their tradition stipulations, modulation in their skill to provide medically and pharmaceutically, very important proteins, and the appliance of animal cells to gene treatment and synthetic organs. This quantity provides the readers an entire overview of current state-of-the-art in Japan. The complaints could be helpful for phone biologists, biochemists, molecular biologists, immunologists, biochemical engineers and different disciplines concerning animal mobilephone tradition, operating in both educational environments or in biotechnology and pharmaceutical industries.
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Additional info for Animal Cell Technology: Challenges for the 21st Century
H. G. H. Y. PARK1 1Biological Production Unit; Central Research Center, Korea Green Cross Corpration, 22 7 Kaigal-Xi, Kiheung-Eup, Yongin-Si, Kyunggi-Do, 449-900, Korea 2DepartmentofAgricultural Chemistry, Korea University, Seoul 136-701, Korea We cultivated the hybridoma producing vWF antibody in a 10L bioreactor in order to investigate the characteristics of two different cultures, intermittent and continuous bleeding. To compare with bleeding culture non-bleeding culture was also performed. 1 x 107 cells/ml at 16th day with 110 mg/l of vwF mAb titer.
Zamboni. A,. , Maddalena, F, Rognoni. , (1994) Production of mouse monoclonal antibodies suing a continuous cell culture fementer and protein G affinity chromatography. Cytotechnology 16, 79-87. Banik, G G , and Heath, C A, (1995) Hybridoma growth and antibody productton as a function of cell density and specific growth rate in perfusion culture, Biotechnol Bioeng 48, 289-300. This page intentionally left blank.
The similarity between mean values indicates that the stirring modes had no significant effect on the number of attached cells. However, the large difference between the standard error values indicates that the distribution ofcells on microcarriers was affected by the stirring modes. TABLE 5 Variation in cell attachment between carriers using different stirring modes (6 hours incubation) The comparison ofthe rate of cell attachment between the ImmobaSil FS carriers, CultiSpher-G (porous microcarriers) and glass beads (solid microcarriers) is shown in Table 6.
Animal Cell Technology: Challenges for the 21st Century by Kouji Ikura, Masaya Nagao, Seiji Masuda, Ryuzo Sasaki