Read e-book online Animal Cell Technology: Basic & Applied Aspects: Proceedings PDF
By Mitsuo Satoh, Shigeru Iida (auth.), Sanetaka Shirahata, Koji Ikura, Masaya Nagao, Akira Ichikawa, Kiichiro Teruya (eds.)
Animal mobilephone expertise is a becoming self-discipline of phone biology which goals not just to appreciate constructions, services and behaviors of differentiated animal cells, but in addition to check their skills for use for commercial and scientific reasons. The aim of animal mobile expertise comprises the clonal growth of differentiated cells, the optimization in their tradition stipulations, modulation in their skill to provide proteins of scientific and pharmaceutical importantance, and the appliance of animal cells to gene remedy, synthetic organs and the creation of practical meals. This quantity offers the readers a whole overview of the current state of the art and may be worthy for these operating in both educational environments or within the biotechnology and pharmaceutical sectors, fairly phone biologists, biochemists, molecular biologists, immunologists, biochemical engineers and all different disciplines on the topic of animal phone culture.
Read Online or Download Animal Cell Technology: Basic & Applied Aspects: Proceedings of the 19th Annual Meeting of the Japanese Association for Animal Cell Technology (JAACT), Kyoto, Japan, September 25-28, 2006 PDF
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Extra info for Animal Cell Technology: Basic & Applied Aspects: Proceedings of the 19th Annual Meeting of the Japanese Association for Animal Cell Technology (JAACT), Kyoto, Japan, September 25-28, 2006
We focused on sericin, one of the main components of virgin silk, as such a mitogenic supplement. We have previously reported that it improves proliferation and survival of all cells tested [1, 2]. We have also shown that sericin improves cultures for the production of adenovirus-based gene-therapy vectors . In this study, we investigated cell proliferation under conditions of growth factor starvaK. Yanagihara, M. Miki, and S. Terada Department of Applied Chemistry and Biotechnology, University of Fukui 3-9-1, Bunkyo, Fukui, 910-8507, Japan A.
The reason for the higher virus titres produced in cultures with no growth factors is that no IGF binding protein is produced. Similarly in culture supplemented with LONG™ R3 IGF-1 a lower binding affinity for IGF BP’s resulted in a fewer complexes for the virus to bind to and consequently inactivated leading to a higher virus titre. Effects of Serum and Growth Factors 23 3 Conclusions In serum free medium we have shown that LONG™ R3 IGF-1 promotes cell proliferation more effectively than IGF-1 and/or insulin in serum free condition.
In the lane of the cells treated with TGF-b, a DNA ladder was observed. On the other hand, no DNA ladder was detected in the cells treated with IL-6 and LIF. This result indicates that IL-6 and LIF did not induce apoptosis, despite strong suppression of cell proliferation. 0 10 LIF concentration (ng/ml) Fig. 1 Effect of cytokines on 2E3 cell proliferation. 1–10 ng/ml (b). 2E3 cells were cultured in serum-free ASF104 medium 50 M. Tanaka et al. 0 10 LIF concentration (ng/ml) Fig. 2 Effect of cytokines on proliferation of 2E3-O cells adapted to serum-free medium.
Animal Cell Technology: Basic & Applied Aspects: Proceedings of the 19th Annual Meeting of the Japanese Association for Animal Cell Technology (JAACT), Kyoto, Japan, September 25-28, 2006 by Mitsuo Satoh, Shigeru Iida (auth.), Sanetaka Shirahata, Koji Ikura, Masaya Nagao, Akira Ichikawa, Kiichiro Teruya (eds.)